Workflows
What is a Workflow?Filters
atavide
is a complete workflow for metagenomics data analysis, including QC/QA, optional host removal, assembly and cross-assembly, and individual read based annotations. We have also built in some advanced analytics including tools to assign annotations from reads to contigs, and to generate metagenome-assembled genomes in several different ways, giving you the power to explore your data!
atavide
is 100% snakemake and conda, so you only need to install the snakemake workflow, and then
...
Exome SAMtools Workflow
Exome Alignment Workflow
Summary
This notebook demonstrates how to retrieve metadata associated to the paper A SARS-CoV-2 cytopathicity dataset generated by high-content screening of a large drug repurposing collection and available in IDR at idr0094-ellinger-sarscov2. Over 300 compounds were used in this investigation. This notebook allows the user to calculate the half maximal inhibitory concentration (IC50) for ...
This is a genomics pipeline to do a single germline sample variant-calling, adapted from GATK Best Practice Workflow.
This workflow is a reference pipeline for using the Janis Python framework (pipelines assistant).
- Alignment: bwa-mem
- Variant-Calling: GATK HaplotypeCaller
- Outputs the final variants in the VCF format.
Resources
This pipeline has been tested using the HG38 reference set, available on Google Cloud Storage through:
Type: Janis
Creators: Richard Lupat, Michael Franklin; Jiaan Yu; Juny Kesumadewi
Submitter: Richard Lupat
A hecatomb is a great sacrifice or an extensive loss. Heactomb the software empowers an analyst to make data driven decisions to 'sacrifice' false-positive viral reads from metagenomes to enrich for true-positive viral reads. This process frequently results in a great loss of suspected viral sequences / contigs.
For information about installation, usage, tutorial etc please refer to the documentation: https://hecatomb.readthedocs.io/en/latest/
Quick start guide
Install Hecatomb from Bioconda ...
Combined workflow for large genome assembly
The tutorial document for this workflow is here: https://doi.org/10.5281/zenodo.5655813
What it does: A workflow for genome assembly, containing subworkflows:
- Data QC
- Kmer counting
- Trim and filter reads
- Assembly with Flye
- Assembly polishing
- Assess genome quality
Inputs:
- long reads and short reads in fastq format
- reference genome for Quast
Outputs:
- Data information - QC, kmers
- Filtered, trimmed reads
- Genome assembly, assembly graph, ...
MetaDEGalaxy: Galaxy workflow for differential abundance analysis of 16s metagenomic data
Assess genome quality; can run alone or as part of a combined workflow for large genome assembly.
- What it does: Assesses the quality of the genome assembly: generate some statistics and determine if expected genes are present; align contigs to a reference genome.
- Inputs: polished assembly; reference_genome.fasta (e.g. of a closely-related species, if available).
- Outputs: Busco table of genes found; Quast HTML report, and link to Icarus contigs browser, showing contigs aligned to a reference ...