CEITEC layer 1 workflow Version 1
Download RO CrateThe second-level complexity workflow is one among a collection of workflows designed to address tasks up to CTF estimation. In addition to the functionalities provided by the layer 0 workflow, this workflow aims to enhance the quality of acquisition images using quality protocols.
Quality control protocols
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Movie max shift: automatic reject those movies whose frames move more than a given threshold.
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Tilt analysis: quality score based in the Power Spectrum Density (astigmatism and tilt)
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CTF consensus: acts as a filter discarding micrographs based on their CTF (limit resolution, defocus, astigmatism, etc.).
Advantages:
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More control of the acquisition quality
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Reduce unnecessary processing time and storage
Version History
Version 2 (earliest) Created 4th Oct 2023 at 14:01 by Daniel Marchan
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Created: 4th Oct 2023 at 14:01
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This document provides a detailed explanation of all the workflows, including their functionalities, problems they address, advantages, disadvantages, implementation requirements, and open points for future versions.
Creator: Daniel Marchan
Submitter: Daniel Marchan
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A set of generic and automatic workflows designed to:
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Run on-the-fly and unattended.
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Maintain robust stability for a wide range of samples.
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Covers steps from movies to CTF estimation (for the moment).
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Monitor the acquisition process and provide user feedback.
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Comprise three proposed workflows, each with an additional layer of complexity.
