Workflow for processing amplicon pool sequencing data without reference sequence. This workflow allows you to reconstruct a sequence from an amplicon pool without a reference sequence. To run this workflow, you need the reads from the pool library you want to analyse in FASTQ format, separated into two files: forward and reverse. You will also need the primers used during sequencing. This workflow creates one or two contigs (depending on whether your primers were used once or twice) and a metadata file containing the length of the contigs, the number of reads mapped to them, and the average, minimum, and maximum coverage depth.