Flashlite-Trinity contains two workflows that run Trinity on the [University of Queensland's HPC, Flashlite](https://rcc.uq.edu.au/flashlite). Trinity performs de novo transcriptome assembly of RNA-seq data by combining three independent software modules Inchworm, Chrysalis and Butterfly to process RNA-seq reads. The algorithm can detect isoforms, handle paired-end reads, multiple insert sizes and strandedness. Users can run Flashlite-Trinity on single samples, or smaller samples requiring <500Gb of memory or staged Trinity which is recommended for global assemblies with multiple sample inputs. Both implementations make use of Singularity containers to install software. Infrastructure\_deployment\_metadata: FlashLite (QRISCloud)