Research Object Crate for ENA SARS-CoV-2 Nanopore Amplicon Sequencing Analysis Workflow

Original URL: https://workflowhub.eu/workflows/104/ro_crate?version=1

A workflow for mapping and consensus generation of SARS-CoV2 whole genome amplicon nanopore data implemented in the Nextflow framework. Reads are mapped to a reference genome using Minimap2 after trimming the amplicon primers with a fixed length at both ends of the amplicons using Cutadapt. The consensus is called using Pysam based on a majority read support threshold per position of the Minimap2 alignment and positions with less than 30x coverage are masked using ‘N’.

Author
David F. Nieuwenhuijse, Alexey Sokolov
License
GPL-3.0

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