3 items tagged with 'DE_NOVO'.
Workflow for processing amplicon pool sequencing data without reference sequence.
This workflow allows you to reconstruct a sequence from an amplicon pool without a reference sequence. To run this workflow, you need the reads from the pool library you want to analyse in FASTQ format, separated into two files: forward and reverse. You will also need the primers used during sequencing. This workflow creates one or two contigs (depending on whether your primers were used once or twice) and a metadata ...
Created: 16th Dec 2025 at 09:38
Stable
Created: 9th Nov 2023 at 09:43, Last updated: 24th Nov 2023 at 15:24
Work-in-progress
Just the cleaning then assembly of all reads. TO explore further follow one of the paths described in "Global view" (WF 0)
Created: 4th Feb 2021 at 09:11, Last updated: 10th Feb 2023 at 10:06
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